I. Foundation of Biochemistry. - 1. Frameworks of Biochemistry. - II. Biomolecules. - 2. Amino Acids and Peptides. - 3. Proteins. - 4. Enzymes. - 5. Carbohydrates. - 6. Lipids and Membranes. - 7. Nucleic Acids. - III. Metabolism. - 8. Introduction to Metabolism. - 9. Bioenergetics. - 10. Carbohydrate Metabolism. - 11. The Citric Acid Cycle. - 12. Electron Transport and Oxidative Phosphorylation. - 13. Lipid Metabolism. - 14 Amino Acid and Nucleotide Metabolism. - 15. Photosynthesis. - IV. Transfer of Genetic Information. - 16. Introduction to Molecular Biology. - 17. ReplicationThe Synthesis of DNA. - 18. TranscriptionThe Synthesis of RNA. - 19. TranslationThe Synthesis of Protein. - Appendix A. AcidBase Calculations. - A. 1. Ionic Strength. - A. 2. pH. - A. 3. HendersonHasselbalch Equation. - A. 4. Buffers. - Appendix B. Principles of Organic Chemistry. - B. 1. Functional Groups. - B. 2. Polar Reactions. - B. 3. Chirality. - B. 4. Optical Isomerism. - B. 5. Conformation and Configuration. - Appendix C. Tools of Biochemistry. - C. 1. Spectrophotometry. - C. 2. Chromatography. - C. 2. 1. Adsorption Chromatography. - C. 2. 2. Ion-Exchange Chromatography. - C. 2. 3. Partition Chromatography. - C. 2. 4. Gel-Filtration Chromatography. - C. 3. Centrifugation. - C3. 1. Analytical Ultracentrifugation. - C. 3. 2. Density Gradient Centrifugation. - C. 4. Electrophoresis. - C. 5. Radioactivity. - C. 6. Recombinant DNA Technology. - C. 6. 1. Selection of Target DNA Fragment. - C. 6. 2. Production of Recombinant DNA. - C. 6. 3. Insertion of Recombinant DNA into Host Cells. - C. 6. 4. Selection of Cells Containing Cloned DNA. - C. 6. 5. Polymerase Chain Reaction. - Appendix D. OxidationReduction Reactions. - D. 1. Half-Reactions. - D. 2. Direction of Redox Reactions. - D. 2. 1. Standard Conditions. - D. 2. 2. Actual Conditions. - Answers to Problems. Language: English