1 Introduction. - 1. 1 The need for this book an active extract what happens next?. - 1. 2 Why is it important to find out the nature of the active components?. - 1. 3 Green chemicals the renewed interest in natural products as sources of bioactive compounds. - 1. 4 What are we doing?. - 1. 5 Bibliography. - 2 Important concepts. - 2. 1 Introduction. - Polarity. - 2. 3 Dilution and concentration factors. - 2. 4 Synergy. - 2. 5 Deterioration decomposition and storage. - 2. 6 Biological variation. - 2. 7 Authentication of material. - 2. 8 Dereplication. - 3 Methods for extraction and sample clean-up. - 3. 1 Introduction. - 3. 2 Factors to be considered in selecting an extraction method. - 3. 3 General extraction methods. - 3. 4 Extraction methods for specific phytochemical groups. - 3. 5 Clean-up techniques to remove unwanted substances. - 3. 6 Bibliography. - 4 Crude fractionation procedures. - 4. 1 Introduction. - 4. 2 Choice of fractionation method. - 4. 3 Common fractionation methods. - 4. 4 Bibliography. - 5 Chromatographic fractionation procedures. - 5. 1 Introduction. - 5. 2 The principles of chromatography. - 5. 3 Physico-chemical processes taking place in chromatography. - 5. 4 Bibliography. - 6 Chromatographic procedures using columns and liquid-liquid systems. - 6. 1 Column chromatography. - 6. 2 Liquid-liquid partition schemes. - 6. 3 Bibliography. - 7 Layer chromatography. - 7. 1 Layer chromatography. - 7. 2 Preparative thin-layer chromatography and paper chromatography. - 7. 3 Bibliography. - 8 Analysis of crude extracts fractions and isolated compounds. - 8. 1 Introduction. - 8. 2 Qualitative analysis of mixtures and isolates. - 8. 3 Quantitative analytical methods. - 8. 4 Biological assays. - 8. 5 Bibliography. - 9 General methods. - 9. 1 Basic techniques in handling extracts. - 9. 2 Isolation of active compounds. - 9. 3Chromatographic procedures. - 9. 4 Basic techniques in thin-layer chromatography. - 9. 5 Bibliography. - 10 Practical exercises in chemical screening and fractionation of crude extracts from plant materials. - 10. 1 Chemical screening. - 10. 2 Exercises in extraction clean-up fractionation and Chromatographicmethods. - Appendix A Recipes for chemical test reagents. Language: English